验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33696-1-AP targets HSPA12A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag36620 Product name: Recombinant human HSPA12A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 516-675 aa of BC156889 Sequence: PPEKLLVKDGTRWCTDVFDKFISADQSVALGELVKRSYTPAKPSQLVIVINIYSSEHDNVSFITDPGVKKCGTLRLDLTGTSGTAVPARREIQTLMQFGDTEIKATAIDIATSKSVKVGIDFLNY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock 70kDa protein 12A |
| 别名 | Heat shock 70 kDa protein 12A, Heat shock protein family A member 12A, KIAA0417 |
| 计算分子量 | 675 aa, 75 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC156889 |
| 基因名称 | HSPA12A |
| Gene ID (NCBI) | 259217 |
| RRID | AB_3743052 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43301 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heat shock protein A12A (HSPA12A) is a distant member of the HSP70 family, which is widely expressed with highest levels in brain, kidney and muscle (PMID: 12552099). HSPA12A encodes a prosurvival pathway against endotoxemic liver injury. Particularly, glycolytic activity in cancer cells is regulated by HSPA12A. HSPA12A modulates glycolysis-derived lactate to affect HMGB1 lactylation and secretion from hepatocytes, by which changes macrophage chemotaxis and activation, and finally affects LI/R injury (PMID: 37441587).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA12A antibody 33696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
