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验证数据展示

  • WB analysis of HEK-293 using 10979-1-AP

    WB analysis of HEK-293 using 10979-1-AP

    WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells..

  • WB analysis using 10979-1-AP

    WB analysis using 10979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10979-1-AP (GRP94 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10979-1-AP

    IHC staining of human breast cancer using 10979-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IF Staining of HEK-293 using 10979-1-AP

    IF Staining of HEK-293 using 10979-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GRP94 antibody (10979-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of MCF-7 using 10979-1-AP

    IP experiment of MCF-7 using 10979-1-AP

    IP result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

  • IF Staining of mouse pancreas using 10979-1-AP

    IF Staining of mouse pancreas using 10979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 10979-1-AP

    FC experiment of MCF-7 using 10979-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug GRP94 Polyclonal antibody (10979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human colon using 10979-1-AP

    IHC staining of human colon using 10979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of mouse brain using 10979-1-AP

    IHC staining of mouse brain using 10979-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10979-1-AP (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • Featured Product
  • KD/KO验证

GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 10979-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue, human appendicitis tissue
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10979-1-AP targets GRP94 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 GRP94 fusion protein Ag1439 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock protein 90kDa beta (Grp94), member 1
别名 HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
计算分子量 96 kDa
观测分子量 94 kDa
GenBank蛋白编号BC009195
基因名称 GRP94
Gene ID (NCBI) 7184
RRIDAB_2119057
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14625
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

实验方案

Product Specific Protocols
WB protocol for GRP94 antibody 10979-1-APDownload protocol
IHC protocol for GRP94 antibody 10979-1-APDownload protocol
IF protocol for GRP94 antibody 10979-1-APDownload protocol
IP protocol for GRP94 antibody 10979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Authors - Na Liu
View Article
human,pigWB

Vet Microbiol

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Authors - Liang Zhang
View Article
ratWB

Mol Med Rep

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Authors - Maojun Liu
View Article
mouseIHC,IF,WB

J Immunol Res

Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis

Authors - Qiaohui Hong
View Article
humanWB

Carcinogenesis

Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis

Authors - Huajie Zong
View Article
humanWB

Exp Brain Res

CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis

Authors - Danyang Wang
View Article