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- KD/KO Validated
GRP94 Polyclonal antibody
GRP94 Polyclonal Antibody for WB, IP, IF/ICC,IF-P, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF/ICC,IF-P, FC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, K-562 cells, NIH/3T3 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse pancreas tissue, MCF-7 cells, HEK-293 cells, human appendicitis tissue |
| Positive FC detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10979-1-AP targets GRP94 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IF/ICC,IF-P, FC, IHC, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | GRP94 fusion protein Ag1439 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Synonyms | Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1 |
| Calculated Molecular Weight | 96 kDa |
| Observed Molecular Weight | 94 kDa |
| GenBank Accession Number | BC009195 |
| Gene Symbol | HSP90B1 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2119057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14625 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRP94 antibody 10979-1-AP | Download protocol |
| IHC protocol for GRP94 antibody 10979-1-AP | Download protocol |
| IF protocol for GRP94 antibody 10979-1-AP | Download protocol |
| IP protocol for GRP94 antibody 10979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neuroinflammation TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. | ||
Vet Microbiol ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. | ||
Mol Med Rep Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats. | ||
J Immunol Res Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis | ||
Carcinogenesis Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis | ||
Exp Brain Res CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis |
