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验证数据展示

  • WB analysis using 67450-1-Ig

    WB analysis using 67450-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67450-1-Ig

    WB analysis of HEK-293 using 67450-1-Ig

    WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for WB, ELISA
Cat No. 67450-1-Ig

产品说明书

CloneNo. 2D3D1
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

HSP90AB1, 2D3D1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67450-1-Ig targets HSP90 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag28379

Product name: Recombinant human HSP90AB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 330-710 aa of BC014485

Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock protein 90kDa alpha (cytosolic), class B member 1
别名 HSP90AB1, 2D3D1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta
计算分子量 723 aa, 83 kDa
观测分子量 83-90 kDa
GenBank蛋白编号BC014485
基因名称 HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2882684
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08238
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

实验方案

Product Specific Protocols
WB protocol for HSP90 antibody 67450-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Virol Sin

Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus

Authors - Bo Wang
  • KD Validated
View Article