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验证数据展示

  • CoIP experiment of mouse brain using 60318-1-Ig (same clone as 60318-1-PBS)

    CoIP experiment of mouse brain using 60318-1-Ig (same clone as 60318-1-PBS)

    Co-IP result of anti-HSP90 (IP:60318-1-Ig, 4ug; Detection:13422-1-AP 1:5000) with mouse brain tissue lysate 2240ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

  • WB analysis using 60318-1-Ig (same clone as 60318-1-PBS)

    WB analysis using 60318-1-Ig (same clone as 60318-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

    IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP90AA1 antibody included
  • IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

    IHC staining of human ovary tumor using 60318-1-Ig (same clone as 60318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSP90AA1 antibody included
  • IF Staining of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

    IF Staining of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

    IP experiment of HepG2 using 60318-1-Ig (same clone as 60318-1-PBS)

    IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug. This data was developed using the same antibody clone with 60318-1-PBS in a different storage buffer formulation.

HSP90 Monoclonal antibody, PBS Only

HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, CoIP, ELISA
Cat No. 60318-1-PBS

产品说明书

CloneNo. 3F11C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, CoIP, ELISA

别名

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSP90AA1 Ready-To-Use IHC Kit. HSP90AA1 primary antibody included.

产品信息

60318-1-PBS targets HSP90 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, CoIP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 HSP90 fusion protein Ag3826 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock protein 90kDa alpha (cytosolic), class A member 1
别名 HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1
计算分子量 853 aa, 90 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC023006
基因名称 HSP90
Gene ID (NCBI) 3320
RRIDAB_2881429
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07900
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.