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验证数据展示

  • WB analysis of mouse brain using 24055-1-AP

    WB analysis of mouse brain using 24055-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24055-1-AP (HSN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24055-1-AP

    IHC staining of human kidney using 24055-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 24055-1-AP

    IHC staining of human kidney using 24055-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 40x lens).

HSN2 Polyclonal antibody

HSN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24055-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HSAN2, HSN2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, MCF-7 cells
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24055-1-AP targets HSN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21184

Product name: Recombinant human HSN2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 86-434 aa of BC130469

Sequence: PGGSPTNWTPEAVVMLGTTASRVTGESCEIQVHPMFEPSQVYSDYRPGLVLPEEAHYFIPQEAVYVAGVHYQARVAEQYEGIPYNSSVLSSPMKQIPEQKPVQGGPTSSSVFEFPSGQAFLVGHLQNLRLDSGLGPGSPLSSISAPISTDATRLKFHPVFVPHSAPAVLTHNNESRSNCVFEFHVHTPSSSSGEGGGILPQRVYRNRQVAVDLNQEELPPQSVGLHGYLQPVTEEKHNYHAPELTVSVVEPIGQNWPIGSPEYSSDSSQITSSDPSDFQSPPPTGGAAAPFGSDVSMPFIHLPQTVLQESPLFFCFPQGTTSQQVLTASFSSGGSALHPQVIGKLPQLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hereditary sensory neuropathy, type II
别名 HSAN2, HSN2
计算分子量 2634 aa, 280 kDa
观测分子量 65 kDa
GenBank蛋白编号BC130469
基因名称 HSN2
Gene ID (NCBI) 378465
RRIDAB_2879417
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9H4A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSN2, also named as WNK1, KDP, KIAA0344, PRKWNK1, P65 and hWNK1, is a serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. It acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. WNK1 may also play a role in actin cytoskeletal reorganization. This antibody detects the 65 kDa protein (Erythrocyte 65 kDa protein, P65).

实验方案

Product Specific Protocols
IHC protocol for HSN2 antibody 24055-1-APDownload protocol
WB protocol for HSN2 antibody 24055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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