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验证数据展示

  • WB analysis using 17333-1-AP (same clone as 17333-1-PBS)

    WB analysis using 17333-1-AP (same clone as 17333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17333-1-AP (HSL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

    IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

HSL Polyclonal antibody, PBS Only

HSL Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17333-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IHC, Indirect ELISA

别名

LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17333-1-PBS targets HSL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag11360

Product name: Recombinant human LIPE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 728-1076 aa of BC070041

Sequence: GNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipase, hormone-sensitive
别名 LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79
计算分子量 117 kDa
观测分子量 84 kDa
GenBank蛋白编号BC070041
基因名称 HSL
Gene ID (NCBI) 3991
RRIDAB_2878386
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05469
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.

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