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验证数据展示

  • WB analysis of human testis using 11970-1-AP

    WB analysis of human testis using 11970-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 11970-1-AP

    IHC staining of human testis using 11970-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

HSFY1 Polyclonal antibody

HSFY1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11970-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HSF2 like, HSF2L, HSFY, HSFY1, HSFY2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 HSFY1 fusion protein Ag2592 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor, Y-linked 1
别名 HSF2 like, HSF2L, HSFY, HSFY1, HSFY2
计算分子量 401 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC055414
基因名称 HSFY1
Gene ID (NCBI) 86614
RRIDAB_2877810
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.

实验方案

Product Specific Protocols
WB protocol for HSFY1 antibody 11970-1-APDownload protocol
IHC protocol for HSFY1 antibody 11970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols