验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32594-1-AP targets HSF2BP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag37637 Product name: Recombinant human HSF2BP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-334 aa of BC000153 Sequence: MGEAGAAEEACRHMGTKEEFVKVRKKDLERLTTEVMQIRDFLPRILNGEVLESFQKLKIVEKNLERKEQELEQLKMDCEHFKARLETVQADNIREKKEKLALRQQLNEAKQQLLQQAEYCTEMGAAACTLLWGVSSSEEVVKAILGGDKALKFFSITGQTMESFVKSLDGDVQELDSDESQFVFALAGIVTNVAAIACGREFLVNSSRVLLDTILQLLGDLKPGQCTKLKVLMLMSLYNVSINLKGLKYISESPGFIPLLWWLLSDPDAEVCLHVLRLVQSVVLEPEVFSKSASEFRSSLPLQRILAMSKSRNPRLQTAAQELLEDLRTLEHNV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock transcription factor 2 binding protein |
| 别名 | MEILB2, Heat shock factor 2-binding protein |
| 计算分子量 | 38 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC000153 |
| 基因名称 | HSF2BP |
| Gene ID (NCBI) | 11077 |
| RRID | AB_3742668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75031 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heat shock factor 2-binding protein (HSF2BP, also known as MEILB2) is a binding protein of HSF2, which is involved in modulating HSF2 activation in testis (PMID: 9651507). Naturally occurring elevated production of HSF2BP in tumors may be a source of cancer-promoting genomic instability and a vulnerable target (PMID: 31960047; 34790765).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HSF2BP antibody 32594-1-AP | Download protocol |
| WB protocol for HSF2BP antibody 32594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
