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验证数据展示

  • WB analysis using 10706-1-AP

    WB analysis using 10706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 10706-1-AP

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 10706-1-AP

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 10706-1-AP

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 10706-1-AP

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

HSF2 Polyclonal antibody

HSF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10706-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, Mouse, rat

应用

WB, IHC, ELISA

别名

Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, A549 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10706-1-AP targets HSF2 in WB, IHC, ELISA applications and shows reactivity with human, Mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, Mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1067

Product name: Recombinant human HSF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-230 aa of BC005329

Sequence: MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIDSGIVKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAQKKNLFQHIVKEPTDNHHHKVIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor 2
别名 Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2
计算分子量 79 kDa
观测分子量 60 kDa
GenBank蛋白编号BC005329
基因名称 HSF2
Gene ID (NCBI) 3298
RRIDAB_2120443
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03933
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSF2 antibody 10706-1-APDownload protocol
IHC protocol for HSF2 antibody 10706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
  • KD Validated
View Article