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HSDL2 Monoclonal antibody

HSDL2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rabbit

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D11G1

Cat No : 68414-1-Ig

Print datasheet

Synonyms

C9orf99, FLJ25855, HSDL2, SDR13C1

验证数据展示

  • WB analysis using 68414-1-Ig

    WB analysis using 68414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68414-1-Ig (HSDL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68414-1-Ig

    IF Staining of HeLa using 68414-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (68414-1-Ig, Clone: 3D11G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig heart tissue, rabbit heart tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68414-1-Ig targets HSDL2 in WB, IF, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, pig, rabbit
Immunogen HSDL2 fusion protein Ag8314 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name hydroxysteroid dehydrogenase like 2
Synonyms C9orf99, FLJ25855, HSDL2, SDR13C1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC004331
Gene Symbol HSDL2
Gene ID (NCBI) 84263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6YN16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

实验方案

Product Specific Protocols
WB protocol for HSDL2 antibody 68414-1-IgDownload protocol
IF protocol for HSDL2 antibody 68414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols