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验证数据展示

  • WB analysis using 68414-1-Ig

    WB analysis using 68414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68414-1-Ig (HSDL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68414-1-Ig

    IF Staining of HeLa using 68414-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (68414-1-Ig, Clone: 3D11G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

HSDL2 Monoclonal antibody

HSDL2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68414-1-Ig

产品说明书

CloneNo. 3D11G1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

C9orf99, FLJ25855, HSDL2, SDR13C1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig heart tissue, rabbit heart tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68414-1-Ig targets HSDL2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, rabbit
免疫原 HSDL2 fusion protein Ag8314 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxysteroid dehydrogenase like 2
别名 C9orf99, FLJ25855, HSDL2, SDR13C1
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC004331
基因名称 HSDL2
Gene ID (NCBI) 84263
RRIDAB_3085130
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6YN16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

实验方案

Product Specific Protocols
WB protocol for HSDL2 antibody 68414-1-IgDownload protocol
IF protocol for HSDL2 antibody 68414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols