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HSDL2 Polyclonal antibody

HSDL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15631-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Short chain dehydrogenase/reductase family 13C member 1, SDR13C1, Hydroxysteroid dehydrogenase-like protein 2, EC:1.-.-.-, C9orf99

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15631-1-AP targets HSDL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 HSDL2 fusion protein Ag7987 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxysteroid dehydrogenase like 2
别名 Short chain dehydrogenase/reductase family 13C member 1, SDR13C1, Hydroxysteroid dehydrogenase-like protein 2, EC:1.-.-.-, C9orf99
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC004331
基因名称 HSDL2
Gene ID (NCBI) 84263
RRIDAB_2120238
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6YN16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

实验方案

Product Specific Protocols
WB protocol for HSDL2 antibody 15631-1-APDownload protocol
IHC protocol for HSDL2 antibody 15631-1-APDownload protocol
IF protocol for HSDL2 antibody 15631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteomics

Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.

Authors - Songlin Zeng
humanIF

Cancer Manag Res

Ectopic expression of HSDL2 is related to cell proliferation and prognosis in breast cancer.

Authors - Bing Dong
humanIHC,WB

Onco Targets Ther

HSDL2 Acts as a Promoter in Pancreatic Cancer by Regulating Cell Proliferation and Lipid Metabolism.

Authors - Anna Han
  • KD Validated
humanWB,FC

Chin Med

Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression.

Authors - Wen-Bei Liu
  • KD Validated
humanIHC

Nan Fang Yi Ke Da Xue Xue Bao

[HSDL2 overexpression promotes rectal cancer progression by regulating cancer cell cycle and promoting cell proliferation]

Authors - Y Cheng
  • KD Validated
mouse,humanWB,IF

Sci Adv

HSDL2 links nutritional cues to bile acid and cholesterol homeostasis

Authors - Nolwenn Samson
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