验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, HeLa cells, MCF-7 cells, U-251 cells |
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15631-1-AP targets HSDL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7987 Product name: Recombinant human HSDL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC004331 Sequence: MLPNTGRLAGCTVFITGASRGIGKAIALKAAKDGANIVIAAKTAQPHPKLLGTIYTAAEEIEAVGGKALPCIVDVRDEQQISAAVEKAIKKFGAYTIAKYGMSMYVLGMAEEFKGEIAVNALWPKTAIHTAAMDMLGGPGIESQCRKVDIIADAAYSIFQKPKSFTGNFVIDENILKEEGIENFDVYAIKPGHPLQPDFFLDEYPEAVSKKVESTGAVPEFKEEKLQLQPKPRSGAVEETFRIVKDSLSDDVVKATQAIYLFELSGEDGGTWFLDLKSKGGNVGYGEPSDQADVVMSMTTDDFVKMFSGKLKPTMAFMSGKLKIKGNMALAIKLEKLMNQMNARL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxysteroid dehydrogenase like 2 |
| 别名 | C9orf99, EC:1.-.-.-, Hydroxysteroid dehydrogenase-like protein 2, SDR13C1, Short chain dehydrogenase/reductase family 13C member 1 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC004331 |
| 基因名称 | HSDL2 |
| Gene ID (NCBI) | 84263 |
| RRID | AB_2120238 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6YN16 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HSDL2 antibody 15631-1-AP | Download protocol |
| IHC protocol for HSDL2 antibody 15631-1-AP | Download protocol |
| WB protocol for HSDL2 antibody 15631-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurotherapeutics Mitochondria-related HSDL2 is a potential biomarker in temporal lobe epilepsy by modulating astrocytic lipid metabolism
| ||
Chin Med Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression.
| ||
Drug Des Devel Ther Semaglutide Reduces Cardiomyocyte Damage Caused by High-Fat Through HSDL2
| ||
Cell Biol Toxicol SIRT5-mediated HSDL2 desuccinylation promotes diabetic-associated cognitive dysfunction via disrupting mitochondrial TRAP1. | ||
BMC Cancer MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell |
