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验证数据展示

  • WB analysis of mouse liver using 10488-1-AP

    WB analysis of mouse liver using 10488-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10488-1-AP (HSD3B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10488-1-AP

    IHC staining of human pancreas cancer using 10488-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse liver using 10488-1-AP

    IP experiment of mouse liver using 10488-1-AP

    IP result of anti-HSD3B7 (IP:10488-1-AP, 4ug; Detection:10488-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

HSD3B7 Polyclonal antibody

HSD3B7 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10488-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

3 beta HSD VII, C(27) 3 beta HSD, HSD3B7, PFIC4, SDR11E3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10488-1-AP targets HSD3B7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0755

Product name: Recombinant human HSD3B7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-369 aa of BC004929

Sequence: MADSAQAQKLVYLVTGGCGFLGEHVVRMLLQREPRLGELRVFDQHLGPWLEELKTGPVRVTAIQGDVTQAHEVAAAVAGAHVVIHTAGLVDVFGRASPKTIHEVNVQGTRNVIEACVQTGTRFLVYTSSMEVVGPNTKGHPFYRGNEDTPYEAVHRHPYPCSKALAEWLVLEANGRKVRGGLPLVTCALRPTGIYGEGHQIMRDFYRQGLRLGGWLFRAIPASVEHGRVYVGNVAWMHVLAARELEQRATLMGGQVYFCYDGSPYRSYEDFNMEFLGPCGLRLVGARPLLPYWLLVFLAALNALLQWLLRPLVLYAPLLNPYTLAVANTTFTVSTDKAQRHFGYEPLFSWEDSRTRTILWVQAATGSAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
别名 3 beta HSD VII, C(27) 3 beta HSD, HSD3B7, PFIC4, SDR11E3
计算分子量 41 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004929
基因名称 HSD3B7
Gene ID (NCBI) 80270
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2F3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSD3B7(3 beta-hydroxysteroid dehydrogenase type 7) is also named as 3-beta-HSD VII,C(27) 3-beta-HSD and belongs to the 3-beta-HSD family. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids and HSD VII is active against four 7-alpha-hydroxylated sterols. This enzyme is expressed predominantly in the liver(PMID:11067870). Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1). It has 2 isoforms produced by alternative splicing.