验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14854-1-AP targets HSD17B7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6639 Product name: Recombinant human HSD17B7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC065246 Sequence: MRKVVLITGASSGIGLALCKRLLAEDDELHLCLACRNMSKAEAVCAALLASHPTAEVTIVQVDVSNLQSVFRASKELKQRFQRLDCIYLNAGIMPNPQLNIKALFFGLFSRKVIHMFSTAEGLLTQGDKITADGLQEVFETNVFGHFILIRELEPLLCHSDNPSQLIWTSSRSARKSNFSLEDFQHSKGKEPYSSSKYATDLLSVALNRNFNQQGLYSNVACPGTALTN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxysteroid (17-beta) dehydrogenase 7 |
| 别名 | 17 beta HSD 7, 3 keto steroid reductase, HSD17B7, PRAP, SDR37C1 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 32-38 kDa |
| GenBank蛋白编号 | BC065246 |
| 基因名称 | HSD17B7 |
| Gene ID (NCBI) | 51478 |
| RRID | AB_10951483 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56937 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for HSD17B7 antibody 14854-1-AP | Download protocol |
| WB protocol for HSD17B7 antibody 14854-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Reprod Biol Endocrinol The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement. | ||
Cell Rep Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics. | ||
Nutrients Malvidin-3-O-Glucoside Ameliorates Cadmium-Mediated Cell Dysfunction in the Estradiol Generation of Human Granulosa Cells | ||
Mol Hum Reprod Semaphorin 4C regulates ovarian steroidogenesis through RHOA/ROCK1-mediated actin cytoskeleton rearrangement | ||
Mol Cell Endocrinol High concentrations of progesterone inhibit the expression of genes related to steroid metabolism in MA-10 Leydig cells |
