验证数据展示
经过测试的应用
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 7 publications below |
| IF | See 4 publications below |
产品信息
13415-1-AP targets HSD17B3 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4081 Product name: Recombinant human HSD17B3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-177 aa of BC034281 Sequence: VRFSRCVLLNYWKVLPKSFLRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGMLPNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxysteroid (17-beta) dehydrogenase 3 |
| 别名 | 17 beta HSD 3, EDH17B3, HSD17B3, SDR12C2 |
| 计算分子量 | 310 aa, 35 kDa |
| GenBank蛋白编号 | BC034281 |
| 基因名称 | HSD17B3 |
| Gene ID (NCBI) | 3293 |
| RRID | AB_2877946 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P37058 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSD17B3 antibody 13415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Endocrinol Transcriptional comparison of testicular adrenal rest tumors with fetal and adult tissues | ||
J Steroid Biochem Mol Biol Features of the fetal gonad in androgen synthesis in the postpubertal testis are preserved in complete androgen insensitivity syndrome due to a novel genetic splice site donor variant in androgen receptor gene intron 1. | ||
Syst Biol Reprod Med Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering | ||
J Endocr Soc Precocious Puberty in a Boy With Bilateral Leydig Cell Tumors due to a Somatic Gain-of-Function LHCGR Variant | ||
Biol Res Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb |