验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, human placenta tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 21 publications below |
| IHC | See 22 publications below |
产品信息
10978-1-AP targets HSD17B2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1411 Product name: Recombinant human HSD17B2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC009581 Sequence: MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hydroxysteroid (17-beta) dehydrogenase 2 |
| 别名 | 17 beta HSD 2, 17βHSD2, 20 alpha HSD, E2DH, EDH17B2, HSD17, HSD17B2, SDR9C2 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC009581 |
| 基因名称 | HSD17B2 |
| Gene ID (NCBI) | 3294 |
| RRID | AB_2279863 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P37059 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| WB protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate Cancer | ||
Phytomedicine Paridis rhizoma total saponins ameliorate abnormal uterine bleeding via regulating Proteus mirabilis 2024-mediated estrogen metabolism in the gut-reproductive axis. | ||
J Hazard Mater Exposure to 1-nitropyrene after weaning induces anxiety-like behavior partially by inhibiting steroid hormone synthesis in prefrontal cortex | ||
Part Fibre Toxicol Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. | ||
J Transl Med Intratumoral localization and activity of 17beta-hydroxysteroid dehydrogenase type 1 in non-small cell lung cancer: a potent prognostic factor. | ||
Fertil Steril Differential expression of steroidogenic enzymes according to endometriosis type. |
