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验证数据展示

  • WB analysis of human placenta using 27830-1-AP

    WB analysis of human placenta using 27830-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 27830-1-AP

    IHC staining of human placenta using 27830-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 27830-1-AP

    IHC staining of human placenta using 27830-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSD17B1 Polyclonal antibody

HSD17B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27830-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

17 beta HSD 1, 20 alpha HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, HSD17, HSD17B1, SDR28C1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27830-1-AP targets HSD17B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag27213

Product name: Recombinant human HSD17B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 191-328 aa of BC104752

Sequence: TAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRSAVGDPELGDPPAAPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxysteroid (17-beta) dehydrogenase 1
别名 17 beta HSD 1, 20 alpha HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, HSD17, HSD17B1, SDR28C1
计算分子量 328 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC104752
基因名称 HSD17B1
Gene ID (NCBI) 3292
RRIDAB_2880985
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14061
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HSD17B1 antibody 27830-1-APDownload protocol
WB protocol for HSD17B1 antibody 27830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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