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HSBP1 Polyclonal antibody

HSBP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10169-2-AP

Print datasheet

Synonyms

HSBP1, HSF1BP

验证数据展示

  • IHC staining of human prostate cancer using 10169-2-AP

    IHC staining of human prostate cancer using 10169-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10169-2-AP (HSBP1 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse brain using 10169-2-AP

    IP experiment of mouse brain using 10169-2-AP

    IP result of anti-HSBP1 (IP:10169-2-AP, 4ug; Detection:10169-2-AP 1:300) with mouse brain tissue lysate 4000ug.

  • WB analysis using 10169-2-AP

    WB analysis using 10169-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 10169-2-AP

    IHC staining of mouse liver using 10169-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 10169-2-AP

    IHC staining of mouse liver using 10169-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, HT-1080 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse liver tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10169-2-AP targets HSBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HSBP1 fusion protein Ag0225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock factor binding protein 1
Synonyms HSBP1, HSF1BP
Calculated Molecular Weight 8.5 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC007515
Gene Symbol HSBP1
Gene ID (NCBI) 3281
RRIDAB_2119501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75506
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSBP1 antibody 10169-2-APDownload protocol
IHC protocol for HSBP1 antibody 10169-2-APDownload protocol
IP protocol for HSBP1 antibody 10169-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia

Authors - Li-Li Chen
View Article