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验证数据展示

  • WB analysis of HT-1080 using 12845-1-AP

    WB analysis of HT-1080 using 12845-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 40x lens).

HS1BP3 Polyclonal antibody

HS1BP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12845-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12845-1-AP targets HS1BP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3880

Product name: Recombinant human HS1BP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC027947

Sequence: MQSPAVLVTSRRLQNAHTGLDLTVPQHQEVRGKMMSGHVEYQILVVTRLAAFKSAKHRPEDVVQFLVSKKYSEIEEFYQKLSSRYAAASLPPLPRKVLFVGESDIRERRAVFNEILRCVSKDAELAGSPELLEFLGTRSPGAAGLTSRDSSVLDGTDSQTGNDEEAFDFFEEQDQVAEEGPPVQSLKGEDAEESLEEEEALDPLGIMRLVSCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HCLS1 binding protein 3
别名 ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3
计算分子量 392 aa, 43 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC027947
基因名称 HS1BP3
Gene ID (NCBI) 64342
RRIDAB_10642005
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53T59
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HS1BP3 antibody 12845-1-APDownload protocol
WB protocol for HS1BP3 antibody 12845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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