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HS1BP3 Polyclonal antibody

HS1BP3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 12845-1-AP

Print datasheet

Synonyms

ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3

验证数据展示

  • WB analysis of HT-1080 using 12845-1-AP

    WB analysis of HT-1080 using 12845-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12845-1-AP targets HS1BP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen HS1BP3 fusion protein Ag3880 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HCLS1 binding protein 3
Synonyms ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC027947
Gene Symbol HS1BP3
Gene ID (NCBI) 64342
RRIDAB_10642005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53T59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HS1BP3 antibody 12845-1-APDownload protocol
IHC protocol for HS1BP3 antibody 12845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols