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HRP-conjugated Mouse Anti-Heavy Chain of Rabbit IgG

Applications

ELISA, WB, Dot blot, IP

Conjugate

HRP

Host

Mouse

Reactivity

Rabbit

Cat No : SA00001-7H

Validation Data Gallery

  • Various serum/plasma lysate were subjected to SDS PAGE followed by western blot with SA00001-7H (HRP-Mouse Anti-Rabbit IgG heavy Chain Specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

    Various serum/plasma lysate were subjected to SDS PAGE followed by western blot with SA00001-7H (HRP-Mouse Anti-Rabbit IgG heavy Chain Specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Jurkat cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-7H (HRP-Mouse Anti-Rabbit IgG heavy Chain Specific antibody) as secondary antibody with dilution 1:15000.

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-7H (HRP-Mouse Anti-Rabbit IgG heavy Chain Specific antibody) as secondary antibody with dilution 1:15000.

Product Information

Concentration 0.2 mg/mL
Applications ELISA, WB, Dot blot, IP
Host Species Mouse
Species Reactivity Rabbit
Physical State Liquid
Suggested Dilution Range 1:5000-1:20,000 for western blotting with ECL substrates
1:5000-1:20,000 for ELISA and Western blotting with chromogenic substrates
Purity Affinity purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3 Store at -20°C.
PreservativeProclin300
Storage Instructions Store at -20°C. Stable for one year after shipment.
Stabilizer0.5% BSA
NoteThis product is for research use only, not for diagnostic or therapeutic use.

Publications

Title

Mol Cancer

CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.

Authors - Hui Zhang
View Article

Antioxidants (Basel)

Oxybaphus himalaicus Mitigates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/MD2 Complex Formation

Authors - Honghong Zhan
View Article

Int J Biol Sci

Timosaponin AIII promotes non-small-cell lung cancer ferroptosis through targeting and facilitating HSP90 mediated GPX4 ubiquitination and degradation

Authors - Cong Zhou
View Article

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang
View Article