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Validation Data Gallery

  • IP-WB detection with RGAR401. Lysate: HeLa cells; IP Primary: Ezrin, 85790-4-RR; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: Ezrin, 85790-4-RR, 1:3000; WB secondary: RGAR401, 1:10000.

    IP-WB detection with RGAR401. Lysate: HeLa cells; IP Primary: Ezrin, 85790-4-RR; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: Ezrin, 85790-4-RR, 1:3000; WB secondary: RGAR401, 1:10000.

  • IP-WB detection with RGAR401. Lysate: Rat Brain cells; IP Primary: DRP1, 81561-1-RR; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: DRP1, 81561-1-RR, 1:50000; WB secondary: RGAR401, 1:10000.

    IP-WB detection with RGAR401. Lysate: Rat Brain cells; IP Primary: DRP1, 81561-1-RR; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: DRP1, 81561-1-RR, 1:50000; WB secondary: RGAR401, 1:10000.

  • IP-WB detection with RGAR401. Lysate: Hela cells; IP Primary: GSR, 18257-1-AP; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: GSR, 18257-1-AP, 1:60000; WB secondary: RGAR401, 1:10000.

    IP-WB detection with RGAR401. Lysate: Hela cells; IP Primary: GSR, 18257-1-AP; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: GSR, 18257-1-AP, 1:60000; WB secondary: RGAR401, 1:10000.

  • IP-WB detection with RGAR401. Lysate: Mouse Brain cells; IP Primary: Synaptophysin, 17785-1-AP; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: Synaptophysin, 17785-1-AP, 1:50000; WB secondary: RGAR401, 1:10000.

    IP-WB detection with RGAR401. Lysate: Mouse Brain cells; IP Primary: Synaptophysin, 17785-1-AP; IP Control: Rabbit IgG, 30000-1-AP; SDS-PAGE loading amount: 20% of IP elution and 30 μg total protein for input; WB Primary: Synaptophysin, 17785-1-AP, 1:50000; WB secondary: RGAR401, 1:10000.

  • Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:100000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:5000 for detection.

    Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:100000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:5000 for detection.

  • Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:100000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:10000 for detection.

    Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:100000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:10000 for detection.

  • Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:100000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:20000 for detection.

    Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:100000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:20000 for detection.

  • Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:20000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:5000 for detection.

    Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:20000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:5000 for detection.

  • Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:20000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:10000 for detection.

    Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:20000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:10000 for detection.

  • Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:20000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:20000 for detection.

    Western blot detection using RGAR401. Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:20000. HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody #RGAR401 was used at 1:20000 for detection.

HRP-Goat Anti-Rabbit IgG Light Chain Specific Recombinant Secondary Antibody

Cat No. RGAR401

Applications

IP-WB, WB, ELISA

Conjugate

HRP

Host

Goat

Reactivity

Rabbit

规格价格库存

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Product Information

Concentration 0.5 mg/mL
Applications IP-WB, WB, ELISA
Host Species Goat
Species Reactivity Rabbit
Physical State Liquid
Suggested Dilution Range 1:5000-1:20000 for ELISA;
1:5000-1:20000 for western blotting or IP-WB with ECL substrates.
Purity The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
Storage Buffer PBS with 50% glycerol, 10 mg/mL BSA, 0.1% ProClin 300, pH 7.4.
Storage Instructions Store at -20°C. Stable for one year after shipment.
ClonalityRecombinant monoclonal
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