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验证数据展示

  • WB analysis using 28763-1-AP

    WB analysis using 28763-1-AP

    MCF-7 cells lysates were subjected to SDS PAGE followed by western blot with 28763-1-AP (HRH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 28763-1-AP

    WB analysis of rat brain using 28763-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 28763-1-AP (HRH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 28763-1-AP

    IHC staining of mouse liver using 28763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28763-1-AP (HRH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HRH1 Polyclonal antibody

HRH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28763-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

H1 R, H1R, HH1R, hisH1, Histamine H1 receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, rat brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28763-1-AP targets HRH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HRH1 fusion protein Ag30464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histamine receptor H1
别名 H1 R, H1R, HH1R, hisH1, Histamine H1 receptor
计算分子量 487 aa, 56 kDa
观测分子量56-60 kDa
GenBank蛋白编号BC060802
基因名称 HRH1
Gene ID (NCBI) 3269
RRIDAB_3086085
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35367
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HRH1 antibody 28763-1-APDownload protocol
IHC protocol for HRH1 antibody 28763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols