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验证数据展示

  • IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

    IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

    WB analysis of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

    IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

    IF Staining of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

HRC Polyclonal antibody, PBS Only

HRC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18142-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18142-1-PBS targets HRC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12794

Product name: Recombinant human HRC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-350 aa of BC069795

Sequence: MTQQLRGDGLGFRNRNNSTGVAGLSEEASAELRHHLHSPRDHPDENKDVSTENGHHFWSHPDREKEDEDVSKEYGHLLPGHRSQDHKVGDEGVSGEEVFAEHGGQARGHRGHGSEDTEDSAEHRHHLPSHRSHSHQDEDEDEVVSSEHHHHILRHGHRGHDGEDDEGEEEEEEEEEEEEASTEYGHQAHRHRGHGSEEDEDVSDGHHHHGPSHRHQGHEEDDDDDDDDDDDDDDDDVSIEYRHQAHRHQGHGIEEDEDVSDGHHHRDPSHRHRSHEEDDNDDDDVSTEYGHQAHRHQDHRKEEVEAVSGEHHHHVPDHRHQGHRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histidine rich calcium binding protein
别名
计算分子量 699 aa, 80 kDa
观测分子量140-150 kDa
GenBank蛋白编号BC069795
基因名称 HRC
Gene ID (NCBI) 3270
RRIDAB_10598013
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23327
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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