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HRAS Polyclonal antibody

HRAS Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15531-1-AP

Print datasheet

Synonyms

C BAS/HAS, C H RAS, C HA RAS1, CTLO, GTPase HRas, H Ras 1, H RASIDX, Ha Ras, HAMSV, HRAS, HRAS1, K RAS, p21ras, RASH1, Transforming protein p21

验证数据展示

  • WB analysis of HeLa using 15531-1-AP

    WB analysis of HeLa using 15531-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15531-1-AP

    IP experiment of HeLa using 15531-1-AP

    IP result of anti-HRAS (IP:15531-1-AP, 4ug; Detection:15531-1-AP 1:300) with HeLa cells lysate 2600 ug.

经过测试的应用

Positive WB detected inHeLa cells, PC-12 cells, MCF-7 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15531-1-AP targets HRAS in WB, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen HRAS fusion protein Ag7704 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Synonyms C BAS/HAS, C H RAS, C HA RAS1, CTLO, GTPase HRas, H Ras 1, H RASIDX, Ha Ras, HAMSV, HRAS, HRAS1, K RAS, p21ras, RASH1, Transforming protein p21
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC006499
Gene Symbol HRAS
Gene ID (NCBI) 3265
RRIDAB_2878147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HRAS antibody 15531-1-APDownload protocol
IP protocol for HRAS antibody 15531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Med Rep

Sirtuin 2 knockdown inhibits cell proliferation and RAS/ERK signaling, and promotes cell apoptosis and cell cycle arrest in multiple myeloma.

Authors - Tianling Ding
View Article
humanWB

Naunyn Schmiedebergs Arch Pharmacol

The potential mechanism of Neu5Gc inducing colorectal cancer based on network pharmacology and experimental validation

Authors - Li-Chao Zhang
View Article