验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, Transfected HEK-293 cells, Raji cells, L02 cells, K-562 cells, mouse lung tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15077-1-AP targets HPS1 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7149 Product name: Recombinant human HPS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-324 aa of BC000175 Sequence: MKCVLVATEGAEVLFYWTDQEFEESLRLKFGQSENEEEELPALEDQLSTLLAPVIISSMTMLEKLSDTYTCFSTENGNFLYVLHLFGECLFIAINGDHTESEGDLRRKLYVLKYLFEVHFGLVTVDGHLIRKELRPPDLAQRVQLWEHFQSLLWTYSRLREQEQCFAVEALERLIHPQLCELCIEALERHVIQAVNTSPERGGEEALHAFLLVHSKLLAFYSSHSASSLRPADLLALILLVQDLYPSESTAEDDIQPSPRRARSSQNIPVQQAWSPHSTGPTGGSSAETETDSFSLPEEYFTPAPSPGDQSSGEDRRKAGGNNS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Hermansky-Pudlak syndrome 1 |
| 别名 | HPS, Hermansky-Pudlak syndrome 1 protein, BLOC-3 complex member HPS1 |
| 计算分子量 | 79 kDa |
| 观测分子量 | 70-79 kDa |
| GenBank蛋白编号 | BC000175 |
| 基因名称 | HPS1 |
| Gene ID (NCBI) | 3257 |
| RRID | AB_10694669 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92902 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HPS1, also named as Hermansky-Pudlak syndrome 1 protein, is a 700 amino acid protein, which is a component of multiple cytoplasmic organelles. HPS1 is Apparently crucial for their normal development and function and involved in intracellular protein sorting.HPS1 is involved in the biogenesis of early melanosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HPS1 antibody 15077-1-AP | Download protocol |
| WB protocol for HPS1 antibody 15077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.
| ||
Am J Respir Cell Mol Biol Dysregulation of Galectin-3: Implications for Hermansky-Pudlak Syndrome Pulmonary Fibrosis. | ||
Clin Exp Metastasis Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1. | ||
Mol Genet Metab In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer. | ||
