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验证数据展示

  • WB analysis using RMX00095

    WB analysis using RMX00095

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00095 (HPRT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using RMX00095

    IHC staining of mouse brain using RMX00095

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00095 (HPRT antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HPRT1 antibody included
  • IF Staining of HeLa using RMX00095

    IF Staining of HeLa using RMX00095

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HPRT antibody (RMX00095) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® HPRT Multi-Recombinant antibody

HPRT Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00095

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells， mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00095 targets HPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 hypoxanthine phosphoribosyltransferase 1
别名 HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase
计算分子量 25 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC000578
基因名称 HPRT1
Gene ID (NCBI) 3251
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP00492
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. Sp2/0 was reported in the literature as an HPRT1-deficient cell line. (PMID:20544509, 28045594, 27379977)

实验方案

Product Specific Protocols
IF protocol for HPRT antibody RMX00095Download protocol
IHC protocol for HPRT antibody RMX00095Download protocol
WB protocol for HPRT antibody RMX00095Download protocol
Standard Protocols
Click here to view our Standard Protocols
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