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Host
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验证数据展示

  • WB analysis using 86049-1-RR (same clone as 86049-1-PBS)

    WB analysis using 86049-1-RR (same clone as 86049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86049-1-RR (HPRT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

    IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included

HPRT Recombinant antibody, PBS Only

HPRT Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86049-1-PBS

产品说明书

CloneNo. 250656C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.

产品信息

86049-1-PBS targets HPRT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HPRT fusion protein Ag7044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxanthine phosphoribosyltransferase 1
别名 HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase
计算分子量 25 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC000578
基因名称 HPRT1
Gene ID (NCBI) 3251
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00492
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. Sp2/0 was reported in the literature as an HPRT1-deficient cell line. (PMID:20544509, 28045594, 27379977)