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验证数据展示

  • WB analysis using 86049-1-RR (same clone as 86049-1-PBS)

    WB analysis using 86049-1-RR (same clone as 86049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86049-1-RR (HPRT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

    IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPRT1 antibody included

HPRT Recombinant monoclonal antibody, PBS Only

HPRT Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86049-1-PBS

产品说明书

CloneNo. 250656C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HPRT1 Ready-To-Use IHC Kit. HPRT1 primary antibody included.

产品信息

86049-1-PBS targets HPRT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7044

Product name: Recombinant human HPRT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-218 aa of BC000578

Sequence: MATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxanthine phosphoribosyltransferase 1
别名 HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase
计算分子量 25 kDa
观测分子量 24-28 kDa
GenBank蛋白编号BC000578
基因名称 HPRT1
Gene ID (NCBI) 3251
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00492
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. Sp2/0 was reported in the literature as an HPRT1-deficient cell line. (PMID:20544509, 28045594, 27379977)

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