HPGD Polyclonal antibody

HPGD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11035-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Eicosanoid/docosanoid dehydrogenase [NAD(+)], EC:1.1.1.141, EC:1.1.1.-, EC:1.1.1.232, 15-PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)]

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HPGD Ready-To-Use IHC Kit. HPGD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11035-1-AP targets HPGD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 HPGD fusion protein Ag1499 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxyprostaglandin dehydrogenase 15-(NAD)
别名 Eicosanoid/docosanoid dehydrogenase [NAD(+)], EC:1.1.1.141, EC:1.1.1.-, EC:1.1.1.232, 15-PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
计算分子量 29 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC018986
基因名称 HPGD
Gene ID (NCBI) 3248
RRIDAB_2120407
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15428
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

实验方案

Product Specific Protocols
WB protocol for HPGD antibody 11035-1-APDownload protocol
IHC protocol for HPGD antibody 11035-1-APDownload protocol
IF protocol for HPGD antibody 11035-1-APDownload protocol
IP protocol for HPGD antibody 11035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Int J Mol Sci

The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia

Authors - Huiyuan Pang
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