验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue |
Positive IP detected in | human placenta tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11035-1-AP targets HPGD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | HPGD fusion protein Ag1499 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | hydroxyprostaglandin dehydrogenase 15-(NAD) |
别名 | Eicosanoid/docosanoid dehydrogenase [NAD(+)], EC:1.1.1.141, EC:1.1.1.-, EC:1.1.1.232, 15-PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)] |
计算分子量 | 29 kDa |
观测分子量 | 25-28 kDa |
GenBank蛋白编号 | BC018986 |
基因名称 | HPGD |
Gene ID (NCBI) | 3248 |
RRID | AB_2120407 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P15428 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for HPGD antibody 11035-1-AP | Download protocol |
IHC protocol for HPGD antibody 11035-1-AP | Download protocol |
IF protocol for HPGD antibody 11035-1-AP | Download protocol |
IP protocol for HPGD antibody 11035-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |