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验证数据展示

  • WB analysis using 12470-1-AP

    WB analysis using 12470-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12470-1-AP (HPCAL4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 12470-1-AP

    IHC staining of human medulloblastoma using 12470-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12470-1-AP (HPCAL4 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of fetal human brain using 12470-1-AP

    IP experiment of fetal human brain using 12470-1-AP

    IP result of anti-HPCAL4 (IP:12470-1-AP, 4ug; Detection:12470-1-AP 1:700) with fetal human brain tissue lysate 3200 ug.

HPCAL4 Polyclonal antibody

HPCAL4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12470-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

hippocalcin like 4, Hippocalcin like protein 4, HLP4, HPCAL4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12470-1-AP targets HPCAL4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3153

Product name: Recombinant human HPCAL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-191 aa of BC030827

Sequence: MGKTNSKLAPEVLEDLVQNTEFSEQELKQWYKGFLKDCPSGILNLEEFQQLYIKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSVTSRGSFEQKLNWAFEMYDLDGDGRITRLEMLEIIEAIYKMVGTVIMMRMNQDGLTPQQRVDKIFKKMDQDKDDQITLEEFKEAAKSDASIVLLLQCDMQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hippocalcin like 4
别名 hippocalcin like 4, Hippocalcin like protein 4, HLP4, HPCAL4
计算分子量 191 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC030827
基因名称 HPCAL4
Gene ID (NCBI) 51440
RRIDAB_2120231
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for HPCAL4 antibody 12470-1-APDownload protocol
IP protocol for HPCAL4 antibody 12470-1-APDownload protocol
WB protocol for HPCAL4 antibody 12470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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