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HOXD9 Polyclonal antibody

HOXD9 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20560-1-AP

Print datasheet

Synonyms

homeobox D9, Homeobox protein Hox 4C, Homeobox protein Hox 5.2, Homeobox protein Hox D9, Hox 4.3, Hox 5.2, HOX4, HOX4C, HOXD9

验证数据展示

  • WB analysis of HeLa using 20560-1-AP

    WB analysis of HeLa using 20560-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20560-1-AP (HOXD9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20560-1-AP

    IF Staining of HeLa using 20560-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20560-1-AP (HOXD9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20560-1-AP targets HOXD9 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox D9
Synonyms homeobox D9, Homeobox protein Hox 4C, Homeobox protein Hox 5.2, Homeobox protein Hox D9, Hox 4.3, Hox 5.2, HOX4, HOX4C, HOXD9
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberNM_014213
Gene Symbol HOXD9
Gene ID (NCBI) 3235
RRIDAB_2878702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HOXD9 antibody 20560-1-APDownload protocol
IF protocol for HOXD9 antibody 20560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

World J Surg Oncol

Homeobox D9 drives the malignant phenotypes and enhances the Programmed death ligand-1 expression in non-small cell lung cancer cells via binding to Angiopoietin-2 promoter

Authors - Jiabei He
View Article