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验证数据展示

  • WB analysis of HeLa using 20560-1-AP

    WB analysis of HeLa using 20560-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20560-1-AP (HOXD9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20560-1-AP

    IF Staining of HeLa using 20560-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20560-1-AP (HOXD9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HOXD9 Polyclonal antibody

HOXD9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20560-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

homeobox D9, Homeobox protein Hox 4C, Homeobox protein Hox 5.2, Homeobox protein Hox D9, Hox 4.3, Hox 5.2, HOX4, HOX4C, HOXD9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20560-1-AP targets HOXD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox D9
别名 homeobox D9, Homeobox protein Hox 4C, Homeobox protein Hox 5.2, Homeobox protein Hox D9, Hox 4.3, Hox 5.2, HOX4, HOX4C, HOXD9
计算分子量 36 kDa
观测分子量 32-37 kDa
GenBank蛋白编号NM_014213
基因名称 HOXD9
Gene ID (NCBI) 3235
RRIDAB_2878702
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28356
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HOXD9 antibody 20560-1-APDownload protocol
IF protocol for HOXD9 antibody 20560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

World J Surg Oncol

Homeobox D9 drives the malignant phenotypes and enhances the Programmed death ligand-1 expression in non-small cell lung cancer cells via binding to Angiopoietin-2 promoter

Authors - Jiabei He
View Article