验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, MDA-MB-453s cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15448-1-AP targets HOXC8 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, chicken, sheep |
| 免疫原 |
CatNo: Ag7675 Product name: Recombinant human HOXC8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC053898 Sequence: MSSYFVNPLFSKYKAGESLEPAYYDCRFPQSVGRSHALVYGPGGSAPGFQHASHHVQDFFHHGTSGISNSGYQQNPCSLSCHGDASKFYGYEALPRQSLYGAQQEASVVQYPDCKSSANTNSSEGQGHLNQNSSPSLMFPWMRPHAPGRRSGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKLPGARDEEKVEEEGNEEEEKEEEEKEENKD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | homeobox C8 |
| 别名 | homeobox C8, Homeobox protein Hox 3A, Homeobox protein Hox C8, Homeobox protein Hox-3A, Homeobox protein Hox-C8 |
| 计算分子量 | 27 aa, 7 kDa |
| 观测分子量 | 33-38 kDa |
| GenBank蛋白编号 | BC053898 |
| 基因名称 | HOXC8 |
| Gene ID (NCBI) | 3224 |
| RRID | AB_2878140 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31273 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOXC8 belongs to highly conserved subgroup of 39 genes housed into four clusters HOXA-HOXD within the superfamily of homeobox genes. HOX genes encode homeodomain- transcription factors that are responsible for regulating morphogenesis through axial patterning during embryogenesis. Homeobox C8 (HOXC8) is a transcription factor preferentially overexpressed in a large percentage of non-small cell lung carcinoma (NSCLC). HOXC8 participates NSCLC development by controlling CASP1 expression and pyroptosis (PMID: 40701951).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXC8 antibody 15448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res N6-methyladenosine-modified circSLCO1B3 promotes intrahepatic cholangiocarcinoma progression via regulating HOXC8 and PD-L1 | ||
iScience DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis | ||
Oncogenesis HOXC8 promotes proliferation and migration through transcriptional up-regulation of TGFβ1 in non-small cell lung cancer.
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iScience Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. | ||
Mol Carcinog Upregulation of MGP by HOXC8 promotes the proliferation, migration, and EMT processes of triple-negative breast cancer.
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