验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, PC-12 cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14321-1-AP targets HOXC4 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 | HOXC4 fusion protein Ag5563 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | homeobox C4 |
| 别名 | HOX3E, HOX3, Homeobox protein Hox-C4, Homeobox protein Hox-3E, cp19 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC050442 |
| 基因名称 | HOXC4 |
| Gene ID (NCBI) | 3221 |
| RRID | AB_2233196 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09017 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The class I homeobox (HOX) family of homeodomain-containing transcription factors has 39 members in mammals. These genes are distributed throughout the genome in four clusters: HOXA (7p15), HOXB (17q21), HOXC (12q13), and HOXD (2q31). HOX genes are known to act as transcription factors, playing an essential regulatory role in the temporal and spatial development of the embryo. HOXC family members are not expressed in normal prostate tissue but increased expression of HOXC genes is commonly detected in prostate cancers. HOXC4 is expressed in GC B cells and HoxC4 expression is specifically up-regulated by the same stimuli that induce AID expression (LPS, CD154 and IL-4). The calculated molecular weight of HOXC4 is 30 kDa. Sometimes higher molecular weight around 39 kDa can also be observed, which may be a modified variant of HOXC4 (PMID: 23178148, 32012197, 35400027).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXC4 antibody 14321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |