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  • Featured Product
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HOXC4 Polyclonal antibody

HOXC4 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14321-1-AP

Print datasheet

Synonyms

cp19, homeobox C4, Homeobox protein CP19, Homeobox protein Hox 3E, Homeobox protein Hox C4, HOX3, HOX3E, HOXC4

验证数据展示

  • WB analysis using 14321-1-AP

    WB analysis using 14321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14321-1-AP (HOXC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 14321-1-AP

    WB analysis of Jurkat using 14321-1-AP

    WB result of HOXC4 antibody (14321-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HOXC4 transfected Jurkat cells.

  • IF Staining of HeLa using 14321-1-AP

    IF Staining of HeLa using 14321-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14321-1-AP (HOXC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, PC-12 cells, U-251 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14321-1-AP targets HOXC4 in WB, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen HOXC4 fusion protein Ag5563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox C4
Synonyms cp19, homeobox C4, Homeobox protein CP19, Homeobox protein Hox 3E, Homeobox protein Hox C4, HOX3, HOX3E, HOXC4
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC050442
Gene Symbol HOXC4
Gene ID (NCBI) 3221
RRIDAB_2233196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09017
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The class I homeobox (HOX) family of homeodomain-containing transcription factors has 39 members in mammals. These genes are distributed throughout the genome in four clusters: HOXA (7p15), HOXB (17q21), HOXC (12q13), and HOXD (2q31). HOX genes are known to act as transcription factors, playing an essential regulatory role in the temporal and spatial development of the embryo. HOXC family members are not expressed in normal prostate tissue but increased expression of HOXC genes is commonly detected in prostate cancers. HOXC4 is expressed in GC B cells and HoxC4 expression is specifically up-regulated by the same stimuli that induce AID expression (LPS, CD154 and IL-4). The calculated molecular weight of HOXC4 is 30 kDa. Sometimes higher molecular weight around 39 kDa can also be observed, which may be a modified variant of HOXC4 (PMID: 23178148, 32012197, 35400027).

实验方案

Product Specific Protocols
WB protocol for HOXC4 antibody 14321-1-APDownload protocol
IF protocol for HOXC4 antibody 14321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.

Authors - Chao Liu
View Article