验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NCCIT cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27002-1-AP targets HOXC13 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | HOXC13 fusion protein Ag25544 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | homeobox C13 |
| 别名 | HOX3G |
| 计算分子量 | 35 kDa, 330 aa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC090850 |
| 基因名称 | HOXC13 |
| Gene ID (NCBI) | 3229 |
| RRID | AB_3669580 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31276 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOXC13 is a member of HOX proteins. The HOX family includes 39 paralogous proteins expressed from the HOXA, HOXB, HOXC, and HOXD gene clusters, and these function early in embryonic development to establish cell and tissue identity and to regulate cell proliferation, differentiation, and survival (PMID: 30974835). Human HOXC13, which is expressed in the hair follicle and nail matrix, is involved in the regulation of keratin gene expression (PMID: 11714694). Mutations in human HOXC13 cause ectodermal dysplasia 9, a severe defect of hair and nails (PMID: 23063621).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXC13 antibody 27002-1-AP | Download protocol |
| IF protocol for HOXC13 antibody 27002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |