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验证数据展示

  • WB analysis of K-562 using 84609-3-RR

    WB analysis of K-562 using 84609-3-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84609-3-RR

    WB analysis using 84609-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84609-3-RR

    Affinity and Kinetic Characterization of 84609-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84609-3-RR against Human HOXB9 were performed. The affinity constant is 40.5 nM.

HOXB9 Recombinant antibody

HOXB9 Recombinant Antibody for WB, ELISA
Cat No. 84609-3-RR

产品说明书

CloneNo. 241866C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HCT 116 cells, HT-29 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84609-3-RR targets HOXB9 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HOXB9 fusion protein Ag34837 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 homeobox B9
别名 HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5
计算分子量 250 aa, 28 kDa
观测分子量30 kDa
GenBank蛋白编号BC015565
基因名称 HOXB9
Gene ID (NCBI) 3219
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17482
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOXB9 is a member of the Homeobox (HOX) gene family, which plays a crucial role in embryonic development and is involved in the regulation of gene expression during this process. In addition to its role in embryogenesis, HOXB9 has been implicated in various cancers, including breast and prostate cancer. It is overexpressed in breast cancer tissue and is regulated by estrogen, playing a critical role in angiogenesis. HOXB9 overexpression has been shown to stimulate three-dimensional colony formation in soft-agar assays, indicating its role in tumorigenesis. Furthermore, HOXB9 binds to the promoters of various tumor growth and angiogenic factors, regulating their expression, and its homeodomain plays a crucial role in transcriptional regulation and tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for HOXB9 antibody 84609-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols