验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells |
| Positive IP detected in | SKOV-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
12613-1-AP targets HOXB7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag3245 Product name: Recombinant human HOXB7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC015345 Sequence: MSSLYYANALFSKYPASSSVFATGAFPEQTSCAFASNPQRPGYGAGSGASFAASMQGLYPGGGGMAGQSAAGVYAAGYGLEPSSFNMHCAPFEQNLSGVCPGDSAKAAGAKEQRDSDLAAESNFRIYPWMRSSGTDRKRGRQTYTRYQTLELEKEFHYNRYLTRRRRIEIAHTLCLTERQIKIWFQNRRMKWKKENKTAGPGTTGQDRAEAEEEEEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | homeobox B7 |
| 别名 | HHO.C1, Homeobox protein HHO.C1, Homeobox protein Hox-2C, Homeobox protein Hox-B7, HOX2C |
| 计算分子量 | 217 aa, 24 kDa |
| 观测分子量 | 24-30 kDa |
| GenBank蛋白编号 | BC015345 |
| 基因名称 | HOXB7 |
| Gene ID (NCBI) | 3217 |
| RRID | AB_2877867 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09629 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOXB7 is a member of the homeobox family genes, which are often dysregulated in various cancer types. Particularly HOXB7 amplification and overexpression correlate with poor prognosis in various cancer such as gastric, pancreatic, and lung cancers. Moreover, HOXB7 is known to contribute to cancer progression by promoting epithelial to mesenchymal transition, anticancer drug resistance, and angiogenesis (PMID: 36659836).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXB7 antibody 12613-1-AP | Download protocol |
| IF protocol for HOXB7 antibody 12613-1-AP | Download protocol |
| IP protocol for HOXB7 antibody 12613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neuroinflammation Gene expression comparison reveals distinct basal expression of HOX members and differential TNF-induced response between brain- and spinal cord-derived microvascular endothelial cells. | ||
Int J Oncol CircRNA hsa_circ_0070934 functions as a competitive endogenous RNA to regulate HOXB7 expression by sponging miR‑1236‑3p in cutaneous squamous cell carcinoma. | ||
Exp Cell Res Long non-coding RNA BBOX1-AS1 exacerbates esophageal squamous cell carcinoma development by regulating HOXB7/β-catenin axis. | ||
Biomed Res Int Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer |
