验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse epididymis tissue, rat colon |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31552-1-AP targets HOXB5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | HOXB5 fusion protein Ag35933 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | homeobox B5 |
| 别名 | HU 1, HOX2A, HHO.C10 |
| 计算分子量 | 29kDa，269aa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | NM_002147.3 |
| 基因名称 | HOXB5 |
| Gene ID (NCBI) | 3215 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P09067 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOXB5 is a member of the Antp homeobox family. HOXB5 is expressed in various tissues, with biased expression in the kidney, colon, and other tissues. HOXB5 plays a crucial role in the development of the lung and gut. It is essential for the proper formation and differentiation of these organs during embryonic development. Hoxb5 exhibits preferential expression in hematopoietic stem cells (HSCs) and uniquely marks the long-term HSCs (LT-HSCs) (PMID: 38324932).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXB5 antibody 31552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |