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验证数据展示

  • IP experiment of HEK-293 using 29978-1-AP

    IP experiment of HEK-293 using 29978-1-AP

    IP result of anti-HOXA9 (IP:29978-1-AP, 4ug; Detection:29978-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

  • IHC staining of human colon using 29978-1-AP

    IHC staining of human colon using 29978-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29978-1-AP (HOXA9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HOXA9 Polyclonal antibody

HOXA9 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 29978-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IP, ELISA

别名

HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29978-1-AP targets HOXA9 in IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32309

Product name: Recombinant human HOXA9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-199 aa of BC010023

Sequence: VYHHHHHHPYVHPQAPVAAAAPDGRYMRSWLEPTPGALSFAGLPSSRPYGIKPEPLSARRGDCPTLDTHTLSLTDYACGSPPVDREKQPSEGAFSENNAENESGGDKPPIDPNNPAAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox A9
别名 HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G
计算分子量 30 kDa
观测分子量36 kDa
GenBank蛋白编号BC010023
基因名称 HOXA9
Gene ID (NCBI) 3205
RRIDAB_3086202
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31269
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homeobox protein Hox-A9 (HOXA9) also known as HOX1G,a member of HOX family belonging to the HOXA cluster, is often studied in acute myeloid leukemia (AML), which is linked to proliferation, differentiation, and progenitor self-renewal maintenance. It is located in nucleoplasm and functions as a critical regulator of hematopoiesis, essential for the maintenance of hematopoietic stem cells and their differentiation into myeloid lineages (PMID: 34743404). Many post-transcriptional events including microRNAs, long non-coding RNAs, and epigenetic modification could also determine HOXA9 protein level and function (PMID: 36376832).

实验方案

Product Specific Protocols
IHC protocol for HOXA9 antibody 29978-1-APDownload protocol
IP protocol for HOXA9 antibody 29978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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