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验证数据展示

  • WB analysis using 27622-1-AP (same clone as 27622-1-PBS)

    WB analysis using 27622-1-AP (same clone as 27622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27622-1-AP (HOXA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27622-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 27622-1-AP (same clone as 27622-1-PBS)

    IP experiment of HEK-293 using 27622-1-AP (same clone as 27622-1-PBS)

    IP result of anti-HOXA5 (IP:27622-1-AP, 4ug; Detection:27622-1-AP 1:500) with HEK-293 cells lysate 1360 ug. This data was developed using the same antibody clone with 27622-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 27622-1-AP (same clone as 27622-1-PBS)

    IF Staining of U2OS using 27622-1-AP (same clone as 27622-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HOXA5 antibody (27622-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27622-1-PBS in a different storage buffer formulation.

HOXA5 Polyclonal antibody, PBS Only

HOXA5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 27622-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

homeobox A5, Homeobox protein Hox 1C, Homeobox protein Hox A5, Homeobox protein Hox-1C, Homeobox protein Hox-A5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27622-1-PBS targets HOXA5 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26362

Product name: Recombinant human HOXA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 64-163 aa of BC013682

Sequence: FGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox A5
别名 homeobox A5, Homeobox protein Hox 1C, Homeobox protein Hox A5, Homeobox protein Hox-1C, Homeobox protein Hox-A5
计算分子量 270 aa, 29 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC013682
基因名称 HOXA5
Gene ID (NCBI) 3202
RRIDAB_3742245
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20719
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HOXA5 (Homeobox A5) is a member of the homeobox gene family, which plays a crucial role in embryonic development, cell differentiation, and tissue patterning (PMID: 31159758). These genes encode transcription factors containing a conserved homeodomain that binds DNA to regulate the expression of target genes involved in morphogenesis and organogenesis.

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