验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue, HEK-293 cells, mouse kidney tissue, human liver tissue, mouse heart tissue, mouse liver tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15457-1-AP targets HOOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7724 Product name: Recombinant human HOOK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 370-718 aa of BC056146 Sequence: TYKRQVVELQNRLSEESKKADKLDFEYKRLKEKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSSRRSYPGHVQPATAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hook homolog 3 (Drosophila) |
| 别名 | Protein Hook homolog 3, h-hook3, hHK3 |
| 计算分子量 | 83 kDa |
| 观测分子量 | 83 kDa |
| GenBank蛋白编号 | BC056146 |
| 基因名称 | HOOK3 |
| Gene ID (NCBI) | 84376 |
| RRID | AB_2120828 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86VS8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| IHC protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| IP protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| WB protocol for HOOK3 antibody 15457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. | ||
Dev Cell The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance. | ||
J Cell Biol Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C. | ||
J Cell Biol Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. | ||
Mol Cell Proteomics Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation | ||
iScience Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.
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