验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32110-1-AP targets HOOK2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | HOOK2 fusion protein Ag37279 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hook homolog 2 (Drosophila) |
| 别名 | Hook Microtubule Tethering Protein 2, hook homolog 2 (Drosophila), HK2, h-hook2, hHK2 |
| 计算分子量 | 83 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | NM_013312 |
| 基因名称 | HOOK2 |
| Gene ID (NCBI) | 29911 |
| RRID | AB_3670194 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96ED9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HOOK2 (hook microtubule-tethering protein 2) is a microtubule-binding protein belonging to the Hook family of proteins.Hook2 normally functions by attaching microtubules to subcellular structures and is involved in a variety of cellular processes, including endosomal/lysosomal processing, Golgi and centrosomal organization, and functional maintenance.Hook2 is Hook2 is required for the assembly of the dynamin-dynamic actin complex and also plays an important role in regulating PAR6α recruitment to centrosomes to bridge microtubules and the aPKC/PAR complex. Therefore, it may be relevant to cell migration, organelle organization, and signaling-related diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HOOK2 antibody 32110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |