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- KD/KO Validated
HOMER1 Polyclonal antibody
HOMER1 Polyclonal Antibody for WB,IP,IF/ICC,IF-P,FC,IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB,IP,IF/ICC,IF-P,FC,IHC,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, human brain tissue, human/mouse brain |
Positive IP detected in | fetal human brain tissue |
Positive IHC detected in | human gliomas tissue, mouse brain tissue, rat brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-12 cells, MCF-7 cells, rat brain tissue |
Positive FC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12433-1-AP targets HOMER1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB,IP,IF/ICC,IF-P,FC,IHC,ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat, zebra finches |
Immunogen | HOMER1 fusion protein Ag3109 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | homer homolog 1 (Drosophila) |
Synonyms | HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, HOMER1, HOMER1A, HOMER1B, HOMER1C, SYN47, Ves 1 |
Calculated Molecular Weight | 40 kDa |
Observed Molecular Weight | 46-48 kDa |
GenBank Accession Number | BC015502 |
Gene Symbol | HOMER1 |
Gene ID (NCBI) | 9456 |
RRID | AB_2295573 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q86YM7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HOMER1 antibody 12433-1-AP | Download protocol |
IHC protocol for HOMER1 antibody 12433-1-AP | Download protocol |
IF protocol for HOMER1 antibody 12433-1-AP | Download protocol |
IP protocol for HOMER1 antibody 12433-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
PLoS Genet Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid. | ||
Aging (Albany NY) Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation.
| ||
CNS Neurosci Ther Acid-sensing Ion Channels Activation and Hypoxia Upregulate Homer1a Expression. | ||
Cell Signal Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c. |