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验证数据展示

  • WB analysis using 83129-5-RR

    WB analysis using 83129-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83129-5-RR (HNRPDL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83129-5-RR

    FC experiment of HeLa using 83129-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRPDL Recombinant antibody (83129-5-RR, Clone:230415F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of U-251 using 83129-5-RR

    IF Staining of U-251 using 83129-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using HNRPDL antibody (83129-5-RR, Clone: 230415F9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HNRPDL Recombinant monoclonal antibody

HNRPDL Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83129-5-RR

产品说明书

CloneNo. 230415F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

230415F9, AU-rich element RNA-binding factor, Heterogeneous nuclear ribonucleoprotein D-like, HNRNP, hnRNP D like

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells, PC-3 cells, fetal human brain tissue, mouse retina tissue
Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83129-5-RR targets HNRPDL in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1046

Product name: Recombinant human HNRPDL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-415 aa of BC007392

Sequence: GSKINASKNQQDDGKMFIGGLSWDTSKKDLTEYLSRFGEVVDCTIKTDPVTGRSRGFGFVLFKDAASVDKVLELKEHKLDGKLIDPKRAKALKGKEPPKKVFVGGLSPDTSEEQIKEYFGAFGEIENIELPMDTKTNERRGFCFITYTDEEPVKKLLESRYHQIGSGKCEIKVAQPKEVYRQQQQQQKGGRGAAAGGRGGTRGRGRGQGQNWNQGFNNYYDQGYGNYNSAYGGDQNYSGYGGYDYTGYNYGNYGYGQGYADYSGQQSTYGKASRGGGNHQNNYQPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein D-like
别名 230415F9, AU-rich element RNA-binding factor, Heterogeneous nuclear ribonucleoprotein D-like, HNRNP, hnRNP D like
计算分子量 46 aa, 4 kDa
观测分子量 38 kDa
GenBank蛋白编号BC007392
基因名称 HNRPDL
Gene ID (NCBI) 9987
RRIDAB_3670833
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO14979
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for HNRPDL antibody 83129-5-RRDownload protocol
WB protocol for HNRPDL antibody 83129-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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