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验证数据展示

  • WB analysis using 10660-2-AP

    WB analysis using 10660-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10660-2-AP (HNRPDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10660-2-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 10660-2-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10660-2-AP (HNRPDL antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HNRPDL Polyclonal antibody

HNRPDL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10660-2-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hnRNP D-like, hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10660-2-AP targets HNRPDL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1046

Product name: Recombinant human HNRPDL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-415 aa of BC007392

Sequence: GSKINASKNQQDDGKMFIGGLSWDTSKKDLTEYLSRFGEVVDCTIKTDPVTGRSRGFGFVLFKDAASVDKVLELKEHKLDGKLIDPKRAKALKGKEPPKKVFVGGLSPDTSEEQIKEYFGAFGEIENIELPMDTKTNERRGFCFITYTDEEPVKKLLESRYHQIGSGKCEIKVAQPKEVYRQQQQQQKGGRGAAAGGRGGTRGRGRGQGQNWNQGFNNYYDQGYGNYNSAYGGDQNYSGYGGYDYTGYNYGNYGYGQGYADYSGQQSTYGKASRGGGNHQNNYQPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein D-like
别名 hnRNP D-like, hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like
计算分子量 46 aa, 4 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC007392
基因名称 HNRPDL
Gene ID (NCBI) 9987
RRIDAB_2120666
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14979
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HNRPDL antibody 10660-2-APDownload protocol
IHC protocol for HNRPDL antibody 10660-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Biochem Funct

Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R).

Authors - Yong-Yang Wu
View Article