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验证数据展示

  • WB analysis using 85422-1-RR (same clone as 85422-1-PBS)

    WB analysis using 85422-1-RR (same clone as 85422-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85422-1-RR (HNRNPM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85422-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85422-1-RR (same clone as 85422-1-PBS)

    IF Staining of HepG2 using 85422-1-RR (same clone as 85422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPM antibody (85422-1-RR, Clone: 242883C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85422-1-PBS in a different storage buffer formulation.

HNRNPM Recombinant monoclonal antibody, PBS Only

HNRNPM Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85422-1-PBS

产品说明书

CloneNo. 242883C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

242883C3, hnRNP M, HNRNPM4, HNRPM, HNRPM4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HNRNPM Ready-To-Use IHC Kit. HNRNPM primary antibody included.

产品信息

85422-1-PBS targets HNRNPM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25474

Product name: Recombinant human HNRNPM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-93 aa of BC000138

Sequence: MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein M
别名 242883C3, hnRNP M, HNRNPM4, HNRPM, HNRPM4
计算分子量 78 kDa
观测分子量 73-77 kDa
GenBank蛋白编号BC000138
基因名称 HNRNPM
Gene ID (NCBI) 4670
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP52272
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPM, also named as NAGR1, is a 730 amino acid protein, which localizes in nucleus. HNRNPM as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. HNRNPM is Involved in splicing. HNRNPM acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. HNRNPM exists three isoforms MW range form 73 kDa and 77 kDa.

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