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HNRNPK Monoclonal antibody

HNRNPK Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A10C8

Cat No : 67708-1-Ig

Print datasheet

Synonyms

CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP

验证数据展示

  • WB analysis using 67708-1-Ig

    WB analysis using 67708-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human colon cancer using 67708-1-Ig

    IHC staining of human colon cancer using 67708-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67708-1-Ig (HNRNPK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPK antibody included
  • WB analysis using 67708-1-Ig

    WB analysis using 67708-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67708-1-Ig (HNRNPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, HuH-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy HNRNPK Ready-To-Use IHC Kit. HNRNPK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67708-1-Ig targets HNRNPK in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen HNRNPK fusion protein Ag30093 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein K
Synonyms CSBP, FLJ41122, hnRNP K, HNRNPK, HNRPK, TUNP
Calculated Molecular Weight 463 aa, 51 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC014980
Gene Symbol HNRNPK
Gene ID (NCBI) 3190
RRIDAB_2882899
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP61978
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability

实验方案

Product Specific Protocols
WB protocol for HNRNPK antibody 67708-1-IgDownload protocol
IHC protocol for HNRNPK antibody 67708-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols