• Featured Product
  • KD/KO Validated

HNRNPK Polyclonal antibody

HNRNPK Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 11426-1-AP

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Synonyms

CSBP, hnRNP K, HNRNPK, HNRPK, TUNP



经过测试的应用

Positive WB detected inMCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPK Ready-To-Use IHC Kit. HNRNPK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11426-1-AP targets HNRNPK in WB, IP, IF, FC, RIP, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, RIP, IHC, CoIP, chIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, deer, pig
Immunogen HNRNPK fusion protein Ag1976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein K
Synonyms CSBP, hnRNP K, HNRNPK, HNRPK, TUNP
Calculated Molecular Weight 463 aa, 51 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC014980
Gene Symbol HNRNPK
Gene ID (NCBI) 3190
RRIDAB_2264314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61978
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability.

实验方案

Product Specific Protocols
WB protocol for HNRNPK antibody 11426-1-APDownload protocol
IHC protocol for HNRNPK antibody 11426-1-APDownload protocol
IF protocol for HNRNPK antibody 11426-1-APDownload protocol
IP protocol for HNRNPK antibody 11426-1-APDownload protocol
FC protocol for HNRNPK antibody 11426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
WB

Nat Commun

Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.

Authors - Wen-Juan Li
  • KD Validated

Nat Commun

PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Authors - Chen-Yu Wang

Nucleic Acids Res

Capturing RNA-protein interaction via CRUIS.

Authors - Ziheng Zhang