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验证数据展示

  • WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    WB analysis using 67701-1-Ig (same clone as 67701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67701-1-Ig (HNRNPF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    IF Staining of HepG2 using 67701-1-Ig (same clone as 67701-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPF antibody (67701-1-Ig, Clone: 2E7A4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67701-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HNRNPF Monoclonal antibody, PBS Only

HNRNPF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67701-1-PBS

产品说明书

CloneNo. 2E7A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

2E7A4, Heterogeneous nuclear ribonucleoprotein F, N-terminally processed, hnRNP F, HNRPF, mcs94 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67701-1-PBS targets HNRNPF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7128

Product name: Recombinant human HNRNPF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-415 aa of BC001432

Sequence: MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein F
别名 2E7A4, Heterogeneous nuclear ribonucleoprotein F, N-terminally processed, hnRNP F, HNRPF, mcs94 1
计算分子量 46 kDa
观测分子量46 kDa
GenBank蛋白编号BC001432
基因名称 HNRNPF
Gene ID (NCBI) 3185
RRIDAB_2882893
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP52597
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

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