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HNRNPA0 Polyclonal antibody

HNRNPA0 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10848-1-AP

Print datasheet

Synonyms

hnRNP A0, HNRNPA0, HNRPA0

验证数据展示

  • IP experiment of mouse brain using 10848-1-AP

    IP experiment of mouse brain using 10848-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:500.

  • WB analysis using 10848-1-AP

    WB analysis using 10848-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10848-1-AP

    IF Staining of HeLa using 10848-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10848-1-AP (HNRNPA0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 10848-1-AP

    WB analysis of HeLa using 10848-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10848-1-AP (HNRNPA0 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, fetal human brain tissue, mouse brain tissue, Jurkat cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10848-1-AP targets HNRNPA0 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen HNRNPA0 fusion protein Ag1312 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein A0
Synonyms hnRNP A0, HNRNPA0, HNRPA0
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC019271
Gene Symbol HNRNPA0
Gene ID (NCBI) 10949
RRIDAB_2117066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

实验方案

Product Specific Protocols
WB protocol for HNRNPA0 antibody 10848-1-APDownload protocol
IF protocol for HNRNPA0 antibody 10848-1-APDownload protocol
IP protocol for HNRNPA0 antibody 10848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article