验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, mouse kidney tissue |
| Positive IHC detected in | rat small intestine tissue, mouse small intestine tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25801-1-AP targets HNF4G in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, chIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22848 Product name: Recombinant human HNF4G protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 327-408 aa of BC105009 Sequence: GGASNDGSHLHHPMHPHLSQDPLTGQTILLGPMSTLVHADQISTPETPLPSPPQGSGQEQYKIAANQASVISHQHLSKQKQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hepatocyte nuclear factor 4, gamma |
| 别名 | Hepatocyte nuclear factor 4-gamma, HNF 4 gamma, HNF-4-gamma, NR2A2, NR2A3 |
| 计算分子量 | 445 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC105009 |
| 基因名称 | HNF4G |
| Gene ID (NCBI) | 3174 |
| RRID | AB_2880242 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14541 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNF4 (hepatocyte nuclear factor 4) is a nuclear receptor protein that has a critical role in hepatocyte differentiation and function (PMID: 12774000). Two isoforms of human HNF4 exist, HNF4-alpha and HNF4-gamma, which are encoded by two separate genes HNF4A and HNF4G respectively (PMID: 7926813). HNF4-gamma has a lower transcription activation potential than HNF4-alpha. HNF4-gamma is expressed in kidney, gut, pancreas, and testis (PMID: 16945327). It has been reported to be involved in cancer progression (PMID: 23896584). This polyclonal antibody is raised against C-terminal 82 amino acids of human HNF4-gamma.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for HNF4G antibody 25801-1-AP | Download protocol |
| IHC protocol for HNF4G antibody 25801-1-AP | Download protocol |
| WB protocol for HNF4G antibody 25801-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Aberrant Activation of a Gastrointestinal Transcriptional Circuit in Prostate Cancer Mediates Castration Resistance. | ||
Protein Cell Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation. | ||
Eur J Pharmacol Hepatocyte nuclear factor 4 gamma (HNF4G) is correlated with poor prognosis and promotes tumor cell growth by inhibiting caspase-dependent intrinsic apoptosis in colorectal cancer.
| ||
Clin Exp Metastasis Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer. | ||
Clin Transl Oncol HNF4G stimulates the development of pancreatic cancer by promoting IGF2BP2 transcription
| ||
Cell Rep MFGE8 links absorption of dietary fatty acids with catabolism of enterocyte lipid stores through HNF4γ-dependent transcription of CES enzymes
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