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验证数据展示

  • WB analysis of L02 using 12533-1-AP (same clone as 12533-1-PBS)

    WB analysis of L02 using 12533-1-AP (same clone as 12533-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

    IHC staining of human renal cell carcinoma using 12533-1-AP (same clone as 12533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNF1B antibody included
  • WB analysis of MDCK using 12533-1-AP (same clone as 12533-1-PBS)

    WB analysis of MDCK using 12533-1-AP (same clone as 12533-1-PBS)

    MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

  • IF Staining of L02 using 12533-1-AP (same clone as 12533-1-PBS)

    IF Staining of L02 using 12533-1-AP (same clone as 12533-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12533-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HNF1B Polyclonal antibody, PBS Only

HNF1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12533-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HNF1B Ready-To-Use IHC Kit. HNF1B primary antibody included.

产品信息

12533-1-PBS targets HNF1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原

CatNo: Ag3232

Product name: Recombinant human HNF1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-310 aa of BC017714

Sequence: MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HNF1 homeobox B
别名 TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta
计算分子量 557 aa, 61 kDa
观测分子量 58-65 kDa
GenBank蛋白编号BC017714
基因名称 HNF1B
Gene ID (NCBI) 6928
RRIDAB_2116758
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35680
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.

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