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HN1L Polyclonal antibody

HN1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 16285-1-AP

Print datasheet

Synonyms

C16orf34, HN1 like protein, HN1L, JPT2, KIAA1426, L11

验证数据展示

  • WB analysis using 16285-1-AP

    WB analysis using 16285-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16285-1-AP (HN1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16285-1-AP targets HN1L in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen HN1L fusion protein Ag9400 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hematological and neurological expressed 1-like
Synonyms C16orf34, HN1 like protein, HN1L, JPT2, KIAA1426, L11
Calculated Molecular Weight 190 aa, 20 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC014438
Gene Symbol HN1L
Gene ID (NCBI) 90861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hematological and neurological expressed 1-like protein (HN1L), also known as Jupiter microtubule-associated homolog 2 (JPT2), belongs to the jupiter family. HN1L is nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein required for NAADP-evoked intracellular calcium release (PubMed:33758061). HN1L enables NAADP to activate Ca2+ release from the endoplasmic reticulum through ryanodine receptors (PubMed:33758062). HN1L was also required for the translocation of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus through the endolysosomal systemHN1L is expressed in liver, kidney, prostate, testis and uterus (PMID: 15094197).

实验方案

Product Specific Protocols
WB protocol for HN1L antibody 16285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols